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sureprint g3 human gene expression 8 × 60k microarray platform  (Agilent technologies)


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    Agilent technologies sureprint g3 human gene expression 8 × 60k microarray platform
    Differentially methylated CpG sites between healthy controls and RSM patients. (a) The purity of the isolated decidual macrophages was detected by FACS. The cells that were positive for CD45 and CD14 were considered decidual macrophages. CD45 + CD14 + cells were also positive for CD68, a pan-macrophage marker. (b) Principal component analysis clearly showed separation between normal macrophages (red square, n = 3) and RSM macrophages (yellow square, n = 3). (c) Heatmap generated from clustering- analysis of <t>microarray</t> data illustrating differentially methylated DNA sites in the RSM patient group relative to the control group. Blue and red represent hypomethylation and hypermethylation, respectively, whereas white indicates no change in methylation relative to the control. Each row represents the beta value of a differentially methylated CpG site and each column represents one sample. (d) Pathways enriched in differentially methylated genes were determined using the GO database.
    Sureprint G3 Human Gene Expression 8 × 60k Microarray Platform, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sureprint+g3+human+gene+expression+8+%C3%97+60k+microarray+platform/pmc10989699-134-28-27?v=Agilent+technologies
    Average 90 stars, based on 1 article reviews
    sureprint g3 human gene expression 8 × 60k microarray platform - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "Upregulation of GPR133 expression impaired the phagocytosis of macrophages in recurrent spontaneous miscarriage"

    Article Title: Upregulation of GPR133 expression impaired the phagocytosis of macrophages in recurrent spontaneous miscarriage

    Journal: Epigenetics

    doi: 10.1080/15592294.2024.2337087

    Differentially methylated CpG sites between healthy controls and RSM patients. (a) The purity of the isolated decidual macrophages was detected by FACS. The cells that were positive for CD45 and CD14 were considered decidual macrophages. CD45 + CD14 + cells were also positive for CD68, a pan-macrophage marker. (b) Principal component analysis clearly showed separation between normal macrophages (red square, n = 3) and RSM macrophages (yellow square, n = 3). (c) Heatmap generated from clustering- analysis of microarray data illustrating differentially methylated DNA sites in the RSM patient group relative to the control group. Blue and red represent hypomethylation and hypermethylation, respectively, whereas white indicates no change in methylation relative to the control. Each row represents the beta value of a differentially methylated CpG site and each column represents one sample. (d) Pathways enriched in differentially methylated genes were determined using the GO database.
    Figure Legend Snippet: Differentially methylated CpG sites between healthy controls and RSM patients. (a) The purity of the isolated decidual macrophages was detected by FACS. The cells that were positive for CD45 and CD14 were considered decidual macrophages. CD45 + CD14 + cells were also positive for CD68, a pan-macrophage marker. (b) Principal component analysis clearly showed separation between normal macrophages (red square, n = 3) and RSM macrophages (yellow square, n = 3). (c) Heatmap generated from clustering- analysis of microarray data illustrating differentially methylated DNA sites in the RSM patient group relative to the control group. Blue and red represent hypomethylation and hypermethylation, respectively, whereas white indicates no change in methylation relative to the control. Each row represents the beta value of a differentially methylated CpG site and each column represents one sample. (d) Pathways enriched in differentially methylated genes were determined using the GO database.

    Techniques Used: Methylation, Isolation, Marker, Generated, Microarray

    Validation of the microarray results via qRT – PCR. the data are presented as the means ± SDs and were analysed by two-sided unpaired Student’s t tests. ** p < 0.01.
    Figure Legend Snippet: Validation of the microarray results via qRT – PCR. the data are presented as the means ± SDs and were analysed by two-sided unpaired Student’s t tests. ** p < 0.01.

    Techniques Used: Microarray, Quantitative RT-PCR



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    Agilent technologies sureprint g3 human gene expression 8 × 60k microarray platform
    Differentially methylated CpG sites between healthy controls and RSM patients. (a) The purity of the isolated decidual macrophages was detected by FACS. The cells that were positive for CD45 and CD14 were considered decidual macrophages. CD45 + CD14 + cells were also positive for CD68, a pan-macrophage marker. (b) Principal component analysis clearly showed separation between normal macrophages (red square, n = 3) and RSM macrophages (yellow square, n = 3). (c) Heatmap generated from clustering- analysis of <t>microarray</t> data illustrating differentially methylated DNA sites in the RSM patient group relative to the control group. Blue and red represent hypomethylation and hypermethylation, respectively, whereas white indicates no change in methylation relative to the control. Each row represents the beta value of a differentially methylated CpG site and each column represents one sample. (d) Pathways enriched in differentially methylated genes were determined using the GO database.
    Sureprint G3 Human Gene Expression 8 × 60k Microarray Platform, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sureprint+g3+human+gene+expression+8+%C3%97+60k+microarray+platform/pmc10989699-134-28-27?v=Agilent+technologies
    Average 90 stars, based on 1 article reviews
    sureprint g3 human gene expression 8 × 60k microarray platform - by Bioz Stars, 2026-07
    90/100 stars
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    Differentially methylated CpG sites between healthy controls and RSM patients. (a) The purity of the isolated decidual macrophages was detected by FACS. The cells that were positive for CD45 and CD14 were considered decidual macrophages. CD45 + CD14 + cells were also positive for CD68, a pan-macrophage marker. (b) Principal component analysis clearly showed separation between normal macrophages (red square, n = 3) and RSM macrophages (yellow square, n = 3). (c) Heatmap generated from clustering- analysis of microarray data illustrating differentially methylated DNA sites in the RSM patient group relative to the control group. Blue and red represent hypomethylation and hypermethylation, respectively, whereas white indicates no change in methylation relative to the control. Each row represents the beta value of a differentially methylated CpG site and each column represents one sample. (d) Pathways enriched in differentially methylated genes were determined using the GO database.

    Journal: Epigenetics

    Article Title: Upregulation of GPR133 expression impaired the phagocytosis of macrophages in recurrent spontaneous miscarriage

    doi: 10.1080/15592294.2024.2337087

    Figure Lengend Snippet: Differentially methylated CpG sites between healthy controls and RSM patients. (a) The purity of the isolated decidual macrophages was detected by FACS. The cells that were positive for CD45 and CD14 were considered decidual macrophages. CD45 + CD14 + cells were also positive for CD68, a pan-macrophage marker. (b) Principal component analysis clearly showed separation between normal macrophages (red square, n = 3) and RSM macrophages (yellow square, n = 3). (c) Heatmap generated from clustering- analysis of microarray data illustrating differentially methylated DNA sites in the RSM patient group relative to the control group. Blue and red represent hypomethylation and hypermethylation, respectively, whereas white indicates no change in methylation relative to the control. Each row represents the beta value of a differentially methylated CpG site and each column represents one sample. (d) Pathways enriched in differentially methylated genes were determined using the GO database.

    Article Snippet: To assess differences in gene expression in decidual macrophages between RSM patients and control individuals, transcriptome profiles were obtained from the two groups of patients using the Agilent SurePrint G3 Human Gene Expression 8 × 60K microarray platform.

    Techniques: Methylation, Isolation, Marker, Generated, Microarray

    Validation of the microarray results via qRT – PCR. the data are presented as the means ± SDs and were analysed by two-sided unpaired Student’s t tests. ** p < 0.01.

    Journal: Epigenetics

    Article Title: Upregulation of GPR133 expression impaired the phagocytosis of macrophages in recurrent spontaneous miscarriage

    doi: 10.1080/15592294.2024.2337087

    Figure Lengend Snippet: Validation of the microarray results via qRT – PCR. the data are presented as the means ± SDs and were analysed by two-sided unpaired Student’s t tests. ** p < 0.01.

    Article Snippet: To assess differences in gene expression in decidual macrophages between RSM patients and control individuals, transcriptome profiles were obtained from the two groups of patients using the Agilent SurePrint G3 Human Gene Expression 8 × 60K microarray platform.

    Techniques: Microarray, Quantitative RT-PCR